Coding

Part:BBa_K4170013:Design

Designed by: Alexandros Giannopoulos Dimitriou   Group: iGEM22_Thessaloniki_Meta   (2022-09-27)


LbuCas13a with 6XHis purification tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1267
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cloning strategy


Source

This plasmid has been constructed from pGJK_His-SUMO-LbuCas13a plasmid, deposited by Jennifer Doudna and her colleagues at Addgene plasmid repository.

References